Figure 2. SOXC gene expression in the limb bud. Sections of hind limbs from E10.5–14.5 wild-type embryos were stained with AB&NFR or hybridized with RNA probes (red signals) and counterstained with DAPI (blue dye), as indicated. All SOXC probes had similar length, GC content, and labeling efficiency, allowing comparison of RNA levels. CM, core mesenchyme; DC, digital condensation; DM, distal mesenchyme; E, ectoderm; EC, epiphyseal cartilage; M, mesenchyme; Pc, perichondrium; PJ, presumptive joint. (A) Coronal sections through hind limb buds. E11.5 Sox11 RNA in situ hybridization image and E13.5 Sox9 RNA in situ hybridization images are composite of several pictures. White lines mark the individual image boundaries. (B) High-magnification views of phalangeal regions boxed in A. (C) Proximal region of E14.5 tibia. All data were reproduced more than three times. Each panel shows representative results.
Figure 2.

SOXC gene expression in the limb bud. Sections of hind limbs from E10.5–14.5 wild-type embryos were stained with AB&NFR or hybridized with RNA probes (red signals) and counterstained with DAPI (blue dye), as indicated. All SOXC probes had similar length, GC content, and labeling efficiency, allowing comparison of RNA levels. CM, core mesenchyme; DC, digital condensation; DM, distal mesenchyme; E, ectoderm; EC, epiphyseal cartilage; M, mesenchyme; Pc, perichondrium; PJ, presumptive joint. (A) Coronal sections through hind limb buds. E11.5 Sox11 RNA in situ hybridization image and E13.5 Sox9 RNA in situ hybridization images are composite of several pictures. White lines mark the individual image boundaries. (B) High-magnification views of phalangeal regions boxed in A. (C) Proximal region of E14.5 tibia. All data were reproduced more than three times. Each panel shows representative results.

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