Knockdown of ALiX in SCs selectively affects female postmating receptivity to other males. (A) Proportion of premated 4-d-old males of different genotypes unable to prevent all females it subsequently mates with from remating (*, P < 0.05; **, P < 0.01, Fisher’s exact test). (B and C) Egg counts (B) and progeny counts (C) from mated females before remating experiments were performed (analyzed by unpaired two-tailed Student’s t test after a Shapiro–Wilk test to confirm normality). (D–F) Mating times (D; **, P < 0.01, Student’s t test), proportion of males that mate within the first 40 min of exposure to females (E) or remate within 40 min of the end of previous mating (F), were also analyzed (using Fisher’s exact test for E and F). Values inside bars show the number of males tested (n). Error bars indicate ±SE. Data shown are from a single representative experiment out of two repeats. DN, dominant negative.