Figure 3.
Figure 3. Dock1 and Elmo2 localize to early cell–cell contacts. (A) Basal focal plane epifluorescence image of single MDCK cells expressing vinculin-GFP and stained with Dock1 antibodies. (B) Basal focal plane confocal image of single MDCK cells expressing vinculin-GFP and stained with Elmo1 and Elmo2 antibodies. (C) Confocal images of confluent MDCK monolayers stained with Dock1 and Elmo2 antibodies at different times after calcium addition and induction of cell–cell adhesion. (D) Confocal imaging of confluent MDCK monolayers stained with Dock1 and Elmo2 antibodies 2.5 h after calcium addition. (E) Epifluorescence images of confluent MDCK monolayer stained with Dock1 and Elmo1 antibodies 2.5 h after calcium addition in cells treated with the indicated siRNAs. Yellow arrowheads indicate the boundary of the cell–cell contact. Bars, 5 µm.

Dock1 and Elmo2 localize to early cell–cell contacts. (A) Basal focal plane epifluorescence image of single MDCK cells expressing vinculin-GFP and stained with Dock1 antibodies. (B) Basal focal plane confocal image of single MDCK cells expressing vinculin-GFP and stained with Elmo1 and Elmo2 antibodies. (C) Confocal images of confluent MDCK monolayers stained with Dock1 and Elmo2 antibodies at different times after calcium addition and induction of cell–cell adhesion. (D) Confocal imaging of confluent MDCK monolayers stained with Dock1 and Elmo2 antibodies 2.5 h after calcium addition. (E) Epifluorescence images of confluent MDCK monolayer stained with Dock1 and Elmo1 antibodies 2.5 h after calcium addition in cells treated with the indicated siRNAs. Yellow arrowheads indicate the boundary of the cell–cell contact. Bars, 5 µm.

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