Figure 7. Warburg Micro syndrome patient cell lines show altered ER morphology. (a) Control and patient fibroblasts were fixed and then stained with antibodies to CLIMP-63 and reticulon 4 (Rtn4). DNA was stained with DAPI (blue in the merged panel). (b) The area of CLIMP-63 as a function of total cell area was measured using ImageJ for 50–70 cells per experiment, for three independent experiments. The mean values are plotted in the bar graph, with error bars indicating the standard deviation of the mean. (c) COS7 cells expressing GFP-Rab18 were treated with control, Rab3GAP (siGAP1+GAP2), or TBC1D20 siRNA duplexes for 72 h. The cells were fixed and then stained with antibodies to CLIMP-63 and Rtn4. DNA was stained with DAPI (blue in the merged panel). The merged panel shows a comparison of GFP-Rab18 and Rtn4 localization to ER tubular networks. Bars are marked in the figure.
Figure 7.

Warburg Micro syndrome patient cell lines show altered ER morphology. (a) Control and patient fibroblasts were fixed and then stained with antibodies to CLIMP-63 and reticulon 4 (Rtn4). DNA was stained with DAPI (blue in the merged panel). (b) The area of CLIMP-63 as a function of total cell area was measured using ImageJ for 50–70 cells per experiment, for three independent experiments. The mean values are plotted in the bar graph, with error bars indicating the standard deviation of the mean. (c) COS7 cells expressing GFP-Rab18 were treated with control, Rab3GAP (siGAP1+GAP2), or TBC1D20 siRNA duplexes for 72 h. The cells were fixed and then stained with antibodies to CLIMP-63 and Rtn4. DNA was stained with DAPI (blue in the merged panel). The merged panel shows a comparison of GFP-Rab18 and Rtn4 localization to ER tubular networks. Bars are marked in the figure.

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