Timing and dynamics of APC/C^Cdc20 substrate degradation. (A) Time from SPB separation to spindle elongation in individual cells with GFP tags on APC/C substrates. Each dot represents a single cell. Starting from the left, sample sizes are: n = 49, 90, 121, 82, and 77 cells. For each strain, the middle bar indicates the median value and error bars indicate the 25th and 75th percentiles. (B) GFP intensity of representative individual cells with tagged APC/C^Cdc20 substrates (from the cell populations analyzed in A). Underlying black lines show the original data, and the colored lines are smoothed traces. The timing of SPB separation and spindle elongation are marked with broken and solid lines, respectively. (C and D) Comparison of different GFP-tagged substrates using SPB separation (C, broken lines) or spindle elongation (D, solid lines) as the timing reference (from the cell populations analyzed in A). In top panels, each line is a smoothed trace of a single cell. A random subset of representative cells is shown for clarity of viewing. Bottom panels show the averaged traces, where unsmoothed traces from all cells were first aligned to the same time reference point, averaged at each time point and then normalized to maximum intensity.