Figure 1.
Metaphase–anaphase transition in cells carrying GFP-tagged APC/C substrates. (A) Network diagram of the metaphase–anaphase transition in budding yeast. (B) GFP-tagged APC/CCdc20 substrates and mCherry-tagged SPBs in cycling cells, at 3-min intervals. Broken circles indicate cells at the onset of SPB separation, and solid circles mark cells at the onset of spindle elongation.