Excess centrosomes disrupt Golgi integrity. (A–C) Time-lapse fluorescence micrographs of centrosomes (centrin::tdTomato; top) and Golgi (GalT::EGFP; bottom) in freely migrating NECs or TECs with one to two or greater than two centrosomes. Red arrows, individual centrosomes. Bars, 10 µm. (D–F) Plots of centrosome perimeter and Golgi area over time from images in A–C. Each graph is a single experiment from several repeats. (G and H) Box and whisker plot (middle bars, mean; boxes, top and bottom quartiles with error bars [SEMs]) of centrosome perimeter and Golgi area from individual live-cell imaging frames (NEC, n = 1,424; TEC 1–2, n = 775; TEC >2, n = 1,075 frames for both graphs). Statistics: Student’s t test; ***, P < 0.0001. see also Videos 5 and 6. (I) Representative images of both centrosomes (centrin::GFP, insets) and Golgi (GalT::tdTomato) in TECs with greater than two centrosomes before and after centrosome ablation. Red arrows, nonablated centrosomes; yellow arrow, ablated centrosome. Bars: (main images) 10 µm; (insets) 5 µm. (J) Representative plot from a single experiment showing centrosome perimeter and Golgi area over time from images in I, before and after centrosome ablation (black arrow, ablation time point). (K) Golgi area of individual TECs with greater than two centrosomes before and after centrosome ablation (n = 7 cells from two experiments). Statistics: one-way Student’s t test; means ± SD. *, P < 0.05.