Ablation of supernumerary centrosomes rescues abnormal centrosome dynamics and endothelial cell migration. (A) Representative images of centrin::GFP-expressing TECs before and after ablation of an extra centrosome. Yellow arrow, ablated centrosome; red arrows, remaining centrosomes. Bar, 10 µm. see also Video 3. (B, left) Diagram showing centrosome distances between centrosomes before ablation (purple and red lines) and centrosome distance of the remaining centrosomal pair after ablation (green line). (right) Actual change in centrosome–centrosome distance over time for a single representative TEC before and after centrosome ablation. (C) Individual centrosome–centrosome distances in the indicated groups (n ≥ 6 cells per condition and three independent experiments). Each dot represents individual centrosome–centrosome distances in the indicated cells. Statistics: Student’s t test; means ± SD. ***, P < 0.0001. (D) Migration tracks of TEC before and after centrosome ablation, combined from three experiments. Each color represents a single cell before and after ablation. Bar, 40 µm. see also Video 4. (E) Mean directional persistence of TECs in the indicated groups, labeled as in D (n ≥ 6 cells per condition). Statistics: one-tailed Student’s t test; error bars show means ± SEM; *, P < 0.05.