Biological roles of SINE1 and SINE2. (A) T-DNA insertion sites of sine1-1, sine1-3, sine2-1, and sine2-2. The left borders of T-DNA insertion sites were confirmed by sequencing and indicated by arrows on SINE1 and SINE2 genomic structures (drawn to scale). Exons are depicted as filled bars, and introns are depicted as lines. DNA fragments encoding the ARM repeats and the TMD-KASH domain are shown in red and orange, respectively. (B) RT-PCR determination of the expression levels of SINE1 and SINE2 in their mutants. Primers amplified the full-length coding sequences are listed in Table S2. (C) Example of measuring nuclear position in guard cells. An ellipse was rendered on a pair of guard cells, and the acute angle between the center of the nucleus and the minor axis (indicated by curved double-headed arrows) was measured. (D) Mean guard cell nuclear positions determined by the angle shown in C. Blue asterisks, P < 0.01 when compared with wild type after blue light treatment; blue circles, P > 0.05 when compared with wild type after blue light treatment; black asterisks, P < 0.01 when compared with wild type without blue light treatment; black circles, P > 0.05 when compared with wild type without blue light treatment. Two-tailed t test was used and n = 172. (E) Quantification of conidiophore formation on the cotyledon adaxial side as a measure of Hpa Noco2 growth on the indicated genotypes. Values are means ± SE of three biological replicates, each with n = 40. Asterisks indicate significant differences to wild type, and circles represent no significant differences to wild type. One-way analysis of variance (α < 0.01; n = 120) followed by Tukey’s honest significant difference test (α < 0.01) was used.