Association of SINE1^1–308 with F-actin filaments. (A) GFP-SINE1^1–308 was transiently coexpressed with RFP-Lifeact (first row) or MAP4-RFP (middle row) in N. benthamiana leaves, showing that GFP-SINE1^1–308 was colocalized with RFP-Lifeact but not MAP4-RFP. GFP-SINE2^1–309 was transiently coexpressed with RFP-Lifeact in N. benthamiana leaves (bottom row), but GFP-SINE2^1–309 was localized to the cytoplasm instead of F-actin fibers labeled by RFP-Lifeact. (B) GFP-SINE1^1–308 and RFP-Lifeact were colocalized in root cells of stably transformed A. thaliana plants. Bars, 10 µm.