FRAP analysis of the interaction between SINE1/2 and AtSUN. Fluorescent protein fusions of SINE1, SINE2, and SUN proteins were transiently expressed in N. benthamiana leaves, and protein mobility was studied by FRAP. (A) Recovery curves of GFP-SINE1 alone or GFP-SINE1 coexpressed with RFP-Flag-AtSUN1 or RFP-Flag-AtSUN1ΔNSUN, respectively. (B) Recovery curves of GFP-SINE1 alone or GFP-SINE1 coexpressed with RFP-Myc-AtSUN2 or RFP-Myc-AtSUN2ΔNSUN, respectively. (C) Recovery curves of GFP-SINE2 alone or GFP-SINE2 coexpressed with RFP-Flag-AtSUN1 or RFP-Flag-AtSUN1ΔNSUN, respectively. (D) Recovery curves of GFP-SINE2 alone or GFP-SINE2 coexpressed with RFP-Myc-AtSUN2 or RFP-Myc-AtSUN2ΔNSUN, respectively. Color-coded asterisks after each curve indicate that the maximum recovery of that curve shows a statistically significant difference when compared with the black curve (P < 0.01, two-tailed t test, n = 35), whereas color-coded circles after each curve indicate no statistical significant difference (P > 0.05, two-tailed t test, n = 35). Error bars represent SEMs.