Co-IP analysis of the SINE3/SINE4/SINE5–AtSUN interactions. (A) Domain organization of SINE3, SINE4, SINE5, and their KASH domain mutants. Diagrams were drawn to scale. Pink, domain N-terminal to the TMD of SINE3; cyan, domain N-terminal to the TMD of SINE4; dark green, domain N-terminal to the TMD of SINE5; yellow, TMD. The numbers above each domain indicate the position of the first and the last amino acid of that domain. The C-terminal 4 aa are indicated in bold. (B) SINE3 interacts with AtSUN1 and AtSUN2 through its KASH domain. (C) AtSUN1 and AtSUN2 interact with SINE3 through their SUN domain. (D) SINE4 interacts with the SUN domain of AtSUN1 through its KASH domain. (E) SINE4 interacts with the SUN domain of AtSUN2 through its KASH domain. (F) SINE5 interacts with the SUN domain of AtSUN1 through its KASH domain. (G) SINE5 interacts with the SUN domain of AtSUN2 through its KASH domain. In B–G, GFP-tagged proteins were immunoprecipitated and detected with anti-GFP antibodies. Myc-tagged proteins were detected with an anti-Myc antibody. The input/IP ratio is 1:9. In D and F, the vertical black lines represent the removal of empty intervening lanes for presentation purposes.