Amino acid sequence alignment of the C-terminal domains of predicted plant KASH proteins. (A–D) Amino acid sequence alignment of SINE1/2 homologues (A), SINE3 homologues (B), SINE4 homologues (C), and SINE5 homologues (D). Full-length protein sequences were used for the alignment, and only the C termini are shown. Aly, A. lyrata; Ata, Aegilops tauschii; Bdi, Brachypodium distachyon; Cru, C. rubella; Csa, Cucumis sativus; Fve, Fragaria vesca; Gma, G. max; Hvu, Hordeum vulgare; Mtr, M. truncatula; Osa, Oryza sativa; Ppa, Physcomitrella patens; Ppe, Prunus persica; Ptr, Populus trichocarpa; Rco, Ricinus communis; Sbi, Sorghum bicolor; Sly, Solanum lycopersicum; Smo, S. moellendorffii; Tur, Triticum urartu; Vvi, V. vinifera; Zma, Zea mays. The numbers after the abbreviations are GI numbers. Locus names of A. thaliana proteins are shown in parentheses. Asterisks indicate that the protein models were corrected according to the predicted TMD-putative KASH domain architecture in the ORFs (see Materials and methods for detail). Numbers at the edges of the alignment indicate the first and last (terminal) amino acids of the domains shown. The Phobius-predicted TMD of the first sequence in each alignment is indicated above the sequence. Filled circles indicate proteins predicted not to have a TMD by Phobius. ClustalX color was assigned to the alignments.