Oncogenic Ras decreases phosphorylation of p130Cas in a p53-dependent manner. (A–C) NIH3T3 cells, iMEFs, and p53^−/− MEFs were infected with a control or Ha-RasV12–expressing retrovirus. (D and E) NIH3T3 cells were infected with a Ha-RasV12–expressing retrovirus together with a control or p53R175H-expressing retrovirus. (A, B, and D) Confocal images of cells stained for F-actin (A) or for paxillin and phosphorylated p130Cas (p-p130Cas; B and D). Bars, 20 µm. (C and E) Levels of phosphorylated p130Cas, phosphorylated FAK (p-FAK), and phosphorylated Src (p-Src) were evaluated by immunoblotting. Blots of phosphorylated p130Cas and p130Cas were quantified and the relative values of p130Cas phosphorylation are shown. (F–H) NIH3T3 cells were infected with Ha-RasV12– and p53R175H-expressing retroviruses together with a control or p130Cas shRNA–expressing retrovirus. (I–K) p53^−/− MEFs were infected with a Ha-RasV12–expressing retrovirus together with a control or p130Cas shRNA–expressing retrovirus. (F and I) The level of p130Cas expression was evaluated by immunoblotting. (G and J) Inverted invasion assays were performed. Images of serial Z-sections (10-µm intervals). Bars, 100 µm. (H and K) Cells invaded 30 µm or more were quantified. Data represent the mean ± SD; n = 5 (H) or n = 3 (K). *, P < 0.05.