Pom1 controls Cdr2 node assembly by modulation of its dynamic exchange on the cortex. (A) Length of the domain occupied by Cdr2-mEGFP nodes in the medial focal plane relative to cell length in wild-type (n = 105) and pom1Δ cells (n = 296). (B) Number of Cdr2-mEGFP nodes in the medial focal plane relative to cell length in wild-type and pom1Δ cells. (C and D) Mean FRAP of Cdr2-mEGFP on the medial cortex in wild-type cells and cells expressing Pom1-chimera (C) or in wild-type and pom1Δ cells (D). Error bars show SDs. Cdr2 t_1/2 in C: pom1⁺ t_1/2 > 90 s; pom1-chimera = 15 s < t_1/2 < 30 s. Cdr2 t_1/2 in D: pom1⁺ t_1/2 = ∼3 min; pom1Δ t_1/2 = ∼3 min. (E) Cdr2-GFP localization in wild-type and pom1Δ cells and in wild-type cells expressing Pom1-chimera. (F) Example of FRAP of Cdr2-mEGFP on the medial cortex in a wild-type cell. The bleached regions are boxed. (G) Example of FRAP of Cdr2-mEGFP on the medial cortex in a cell expressing Pom1-chimera. The bleached regions are boxed. P, prebleach; B, bleach. Bars, 4 µm.