Figure 4. Mid1 contributes to Cdr2 N-terminal clustering. (A) Medial plane epifluorescence images of Cdr2-mEGFP, Cdr2N-Kcc4C–GFP chimera, and Cdr2FF*-mEGFP in wild-type (wt) cells and mid1Δ400–450 mutant deficient for Mid1 interaction with Cdr2. Bar, 5 µm. (B) Coimmunoprecipitation assays between mEGFP- and myc12-tagged Cdr2 or GFP- and myc12-tagged Cdr2N-Kcc4C. Immunoprecipitations were performed with anti-GFP mAb. Inputs and immunoprecipitation (IP) samples were probed with anti-GFP and anti-myc mAbs. Molecular masses are indicated. WB, Western blot.
Figure 4.

Mid1 contributes to Cdr2 N-terminal clustering. (A) Medial plane epifluorescence images of Cdr2-mEGFP, Cdr2N-Kcc4C–GFP chimera, and Cdr2FF*-mEGFP in wild-type (wt) cells and mid1Δ400–450 mutant deficient for Mid1 interaction with Cdr2. Bar, 5 µm. (B) Coimmunoprecipitation assays between mEGFP- and myc12-tagged Cdr2 or GFP- and myc12-tagged Cdr2N-Kcc4C. Immunoprecipitations were performed with anti-GFP mAb. Inputs and immunoprecipitation (IP) samples were probed with anti-GFP and anti-myc mAbs. Molecular masses are indicated. WB, Western blot.

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