Figure 6. Conditional dendritic transport of CLN2 mRNA. (A and B) ID3-containing CLN2 mRNA was not targeted to dendrites of sympathetic neurons under basal conditions. (C–F) CLN2 mRNA was delivered to dendrites after β-AR activation with agonist isoproterenol (C and D) but not after α-adrenergic activation with agonist phenylephrine (E and F). Number of cells analyzed: (A and B) 19 cells, 72 dendrites; (C and D) 17 cells, 63 dendrites; (E and F) 18 cells, 66 dendrites. Bar, 50 µm. (G) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of RNA levels in the basal state with RNA levels after adrenergic activation): comparison with isoproterenol (C and D), P < 0.001 for all interval points; comparison with phenylephrine (E and F), P > 0.8 for interval points 50 µm. Error bars indicate SEM.
Figure 6.

Conditional dendritic transport of CLN2 mRNA. (A and B) ID3-containing CLN2 mRNA was not targeted to dendrites of sympathetic neurons under basal conditions. (C–F) CLN2 mRNA was delivered to dendrites after β-AR activation with agonist isoproterenol (C and D) but not after α-adrenergic activation with agonist phenylephrine (E and F). Number of cells analyzed: (A and B) 19 cells, 72 dendrites; (C and D) 17 cells, 63 dendrites; (E and F) 18 cells, 66 dendrites. Bar, 50 µm. (G) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of RNA levels in the basal state with RNA levels after adrenergic activation): comparison with isoproterenol (C and D), P < 0.001 for all interval points; comparison with phenylephrine (E and F), P > 0.8 for interval points 50 µm. Error bars indicate SEM.

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