Figure 4. Mutant BC1 RNA in conditional dendritic delivery. (A–D) WT BC1 RNA (G35=C41) is constitutively targeted to dendrites of sympathetic neurons in culture (Muslimov et al., 1997, 2006, 2011). (A and C) In contrast, mutant A35•G41 BC1 RNA and mutant G35•G41 BC1 RNA were not transported under basal conditions. (B and D) Both mutant BC1 RNA forms were, however, delivered to dendrites upon K+ depolarization. Number of cells analyzed: (A) 18 cells, 57 dendrites; (B) 17 cells, 56 dendrites; (C) 16 cells, 52 dendrites; (D) 16 cells, 44 dendrites. Bar, 50 µm. (E) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of levels of mutant BC1 RNAs in the basal state with levels after K+ depolarization): BC1 RNA A35•G41 (A and B), P < 0.001 for all interval points; BC1 RNA G35•G41 (C and D), P < 0.001 for all interval points. Error bars indicate SEM.
Figure 4.

Mutant BC1 RNA in conditional dendritic delivery. (A–D) WT BC1 RNA (G35=C41) is constitutively targeted to dendrites of sympathetic neurons in culture (Muslimov et al., 1997, 2006, 2011). (A and C) In contrast, mutant A35•G41 BC1 RNA and mutant G35•G41 BC1 RNA were not transported under basal conditions. (B and D) Both mutant BC1 RNA forms were, however, delivered to dendrites upon K+ depolarization. Number of cells analyzed: (A) 18 cells, 57 dendrites; (B) 17 cells, 56 dendrites; (C) 16 cells, 52 dendrites; (D) 16 cells, 44 dendrites. Bar, 50 µm. (E) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of levels of mutant BC1 RNAs in the basal state with levels after K+ depolarization): BC1 RNA A35•G41 (A and B), P < 0.001 for all interval points; BC1 RNA G35•G41 (C and D), P < 0.001 for all interval points. Error bars indicate SEM.

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