![Figure 3. Dendritic transport of ID3- and ID4-chimeric mRNAs in stimulated neurons. (A–D) Sympathetic neurons in culture were K+ depolarized before RNA microinjection. Under these conditions, ID3 (A and B)- and ID4 (C and D)-chimeric α-tubulin mRNAs were delivered to distal dendrites. Number of cells analyzed: (A and B) 16 neurons, 55 dendrites; (C and D) 18 neurons, 60 dendrites. Bar, 50 µm. (E) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of RNA levels in the basal, nonstimulated state [Fig. 2] with RNA levels after K+ depolarization): ID3-chimeric α-tubulin mRNA (A and B), P < 0.001 for all interval points; ID4-chimeric α-tubulin mRNA (C and D), P < 0.001 for all interval points. Error bars indicate SEM.](https://cdn.rupress.org/rup/content_public/journal/jcb/205/4/10.1083_jcb.201310045/5/jcb_201310045r_fig3.jpeg?Expires=1767671354&Signature=gO6VusrTyOjho56uAQ8QvxUjHjsBOShopuVPe6ntANyR4BEkAM4lklLYaImCHmkjG-wylH~yoY-z-j6BhWspQA6mOpvth387JyZua~Ql12-xZCrlbpgsMQBAzFY211L5AitU3Qxk3wksOmR0erdzI6nC4nz-c8zZplrx2UkNO3zvunNSq4ZRf1SJU4zwnBOcEh6DcVhMm3114q9Z3uPchNWHJAItvMMaI4J8t1gltHCBeQy6hVvqjk72KByFBoIFa7y~TJ0drBRwStE74MxAc2FuaAFXUF2v~w-S8itk139djqj~uLuuO96Td1EeH6Hb~vWiCM-ERJRFQPCwpVyM1A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Dendritic transport of ID3- and ID4-chimeric mRNAs in stimulated neurons. (A–D) Sympathetic neurons in culture were K+ depolarized before RNA microinjection. Under these conditions, ID3 (A and B)- and ID4 (C and D)-chimeric α-tubulin mRNAs were delivered to distal dendrites. Number of cells analyzed: (A and B) 16 neurons, 55 dendrites; (C and D) 18 neurons, 60 dendrites. Bar, 50 µm. (E) Quantitative analysis. One-way ANOVA, Dunnett’s post hoc analysis (comparison of RNA levels in the basal, nonstimulated state [Fig. 2] with RNA levels after K+ depolarization): ID3-chimeric α-tubulin mRNA (A and B), P < 0.001 for all interval points; ID4-chimeric α-tubulin mRNA (C and D), P < 0.001 for all interval points. Error bars indicate SEM.
