Figure 2. The FNIII repeats of TNX molecule inhibit the EMT induced by the FBG domain in mammary epithelial cells. (A) Phase-contrast images of NMuMG cells cultured for 48 h either in the presence of soluble recombinant human TGF-β1 (2 ng/ml) or after seeding onto noncoated dishes or dishes coated (111 pmol/cm2) with the TNXΔEΔF fragment, the FBG domain, or both recombinant proteins. Bars, 30 µm. (B) F-actin direct fluorescence and E-cadherin indirect immunofluorescence were performed in NMuMG cells cultured as described in A. Bars, 15 µm.
Figure 2.

The FNIII repeats of TNX molecule inhibit the EMT induced by the FBG domain in mammary epithelial cells. (A) Phase-contrast images of NMuMG cells cultured for 48 h either in the presence of soluble recombinant human TGF-β1 (2 ng/ml) or after seeding onto noncoated dishes or dishes coated (111 pmol/cm2) with the TNXΔEΔF fragment, the FBG domain, or both recombinant proteins. Bars, 30 µm. (B) F-actin direct fluorescence and E-cadherin indirect immunofluorescence were performed in NMuMG cells cultured as described in A. Bars, 15 µm.

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