Figure 1.
Recombinant TNX fragments differentially regulate EMT in mammary epithelial cells. (A) The recombinant TNX fragments (solid lines) are depicted below the schematic diagram representing the full-length TNX monomer. (B) Phase-contrast images of NMuMG cells cultured for 48 h either in the presence of soluble recombinant human TGF-β1 (2 ng/ml) or after seeding onto noncoated dishes or dishes coated (111 pmol/cm2) with recombinant full-length TNX, TNXΔEΔF fragment, or FBG domain. Bars, 30 µm. (C) F-actin direct fluorescence and E-cadherin indirect immunofluorescence were performed in NMuMG cells cultured as described in B. Bars, 15 µm.
