Figure 5. Vps4-mediated ESCRT-III disassembly controls ILV biogenesis in vivo. (A) Electron tomography of cryofixed WT cells overexpressing Vps21 (TDH3-VPS21) and the indicated mutants. 2D slices from tomographic reconstructions and models from 400-nm sections are shown. Limiting MVB membrane (yellow), ILVs (red), nuclear envelope (blue), and class E–like structures (green) are shown. Bars, 150 nm. Arrowheads point to enlarged budding profiles in the snf7*, vps2* double mutants. (B) Size distribution (in 10-nm steps) of ILV diameters in WT cells and the indicated mutants. (C) Mean number of ILVs/MVB for ≥10 fully reconstructed MVBs from WT cells and the indicated mutants. Error bars indicate the SDs. *, P < 0.05; ***, P < 0.001.
Figure 5.

Vps4-mediated ESCRT-III disassembly controls ILV biogenesis in vivo. (A) Electron tomography of cryofixed WT cells overexpressing Vps21 (TDH3-VPS21) and the indicated mutants. 2D slices from tomographic reconstructions and models from 400-nm sections are shown. Limiting MVB membrane (yellow), ILVs (red), nuclear envelope (blue), and class E–like structures (green) are shown. Bars, 150 nm. Arrowheads point to enlarged budding profiles in the snf7*, vps2* double mutants. (B) Size distribution (in 10-nm steps) of ILV diameters in WT cells and the indicated mutants. (C) Mean number of ILVs/MVB for ≥10 fully reconstructed MVBs from WT cells and the indicated mutants. Error bars indicate the SDs. *, P < 0.05; ***, P < 0.001.

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