Figure 4. The MIMs of Vps2 and Snf7 couple ESCRT-III disassembly to ILV biogenesis. (A) Sections 1–7 show schematic representation of ESCRT-III complexes, the corresponding subcellular fractionation, and live-cell microscopy using GFP-CPS and FM4-64. Bar, 5 µM. Membrane fractions (M) and cytoplasmic fraction (C) of the indicated mutants were analyzed by SDS-PAGE and Western blotting. The uncut films are shown in Fig. S3 A. V, vacuole; E, class E compartment; DIC, differential interference contrast. (B) Transmission EM of cryofixed WT cells and snf7-MIM1, vps2* mutants with class E compartments. Bar, 250 nm. (C) GFP-CPS–sorting phenotypes of the tested strains with different chimeric ESCRT-III complexes.
Figure 4.

The MIMs of Vps2 and Snf7 couple ESCRT-III disassembly to ILV biogenesis. (A) Sections 1–7 show schematic representation of ESCRT-III complexes, the corresponding subcellular fractionation, and live-cell microscopy using GFP-CPS and FM4-64. Bar, 5 µM. Membrane fractions (M) and cytoplasmic fraction (C) of the indicated mutants were analyzed by SDS-PAGE and Western blotting. The uncut films are shown in Fig. S3 A. V, vacuole; E, class E compartment; DIC, differential interference contrast. (B) Transmission EM of cryofixed WT cells and snf7-MIM1, vps2* mutants with class E compartments. Bar, 250 nm. (C) GFP-CPS–sorting phenotypes of the tested strains with different chimeric ESCRT-III complexes.

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