Figure 3.
Figure 3. Stromal domains of cpTatC contact the stromal domain of Tha4; lumenal domains of cpTatC contact the lumen-proximal domain of Tha4. Thylakoid membranes containing radiolabeled cpTatC single Cys variants were incubated with unlabeled Tha4 single Cys variants and SpF16 or with mock translation extract (−) as in Fig. 1 and subjected to disulfide cross-linking (see Materials and methods). Samples were analyzed by nonreducing SDS-PAGE/fluorography. (A) cpTatC with Cys residues in transmembrane domains. (B) cpTatC with Cys residues in stroma-exposed and lumen-exposed domains.

Stromal domains of cpTatC contact the stromal domain of Tha4; lumenal domains of cpTatC contact the lumen-proximal domain of Tha4. Thylakoid membranes containing radiolabeled cpTatC single Cys variants were incubated with unlabeled Tha4 single Cys variants and SpF16 or with mock translation extract (−) as in Fig. 1 and subjected to disulfide cross-linking (see Materials and methods). Samples were analyzed by nonreducing SDS-PAGE/fluorography. (A) cpTatC with Cys residues in transmembrane domains. (B) cpTatC with Cys residues in stroma-exposed and lumen-exposed domains.

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