ATG37/ACBD5 is dispensable for aggrephagy and nonselective autophagy. (A) ACBD5 is not required for the autophagy of Htt-Q72 aggregates in HeLa cells. Cells expressed EGFP-Htt-Q72. Arrows point to the EGFP-Htt-Q72 aggregates. Bar, 10 µm. (B) Quantitation of data in A. The percentage of cells with EGFP-Htt-Q72 aggregates is shown as the mean + SD (error bars; n ≥ 50; *, P < 0.05 vs. siATG7). (C) ACBD5 does not affect SQSTM1 degradation and LC3 lipidation. Short, short exposure; Long, long exposure; β-Actin, loading control. (D) ACBD5 is dispensable for LC3-positive vesicle formation in HeLa cells expressing fluorescently tagged LC3. Cells were treated with 20 µM chloroquine in EBSS medium for 3 h and permeabilized with 0.025% digitonin to enrich for the vesicle-bound LC3 (indicated by arrows). Bar, 10 µm. (E) Quantitation of data in D. The percentage of cells with the vesicle-bound LC3 is shown as the mean + SD (error bars; n ≥ 75; *, P < 0.05 vs. siATG16L1).