ACBS/30BS is required for Atg37 localization and phagophore formation. (A) The ACBS/30BS is required for proper localization of Atg37. Arrows point to the peroxisome clusters with accumulation of Atg37-GFP in the middle. Bar, 5 µm. (B) Quantitation of data in A. The data shown are from a single representative experiment out of three repeats. (C and D) The ACBS/30BS of Atg37 is also necessary for phagophore formation (C) and pexophagy (D). Methanol-grown cells were adapted to glucose medium (C) or ethanol medium without nitrogen (D; SE-N). Arrows point to the MIPA. Bar, 5 µm.