Figure 1. Atg37 is an integral PMP that binds Pex3. (A) Pex3 coimmunoprecipitates with Atg37. IP, immunoprecipitate. Cells were induced in methanol medium for 4 h. (B) Atg37ΔC binds Pex3ΔN in vitro. I, input; U, unbound; P, pull-down. (C) Atg37 is an integral membrane protein. PNS, postnuclear supernatant; 27S, 27,000 g supernatant; 27P, 27,000 g pellet; S, 200,000 g supernatant; P, 200,000 g pellet. (D) Atg37 localizes to the peroxisomal membranes (in WT) or ER-associated peroxisome remnants labeled with Pex3-mRFP (in Δpex19). DIC, differential interference contrast. Bar, 5 µm. (E) The TMD is essential for peroxisomal localization of Atg37. SKL, Ser-Lys-Leu, a peroxisomal targeting signal. Bar, 5 µm.
Figure 1.

Atg37 is an integral PMP that binds Pex3. (A) Pex3 coimmunoprecipitates with Atg37. IP, immunoprecipitate. Cells were induced in methanol medium for 4 h. (B) Atg37ΔC binds Pex3ΔN in vitro. I, input; U, unbound; P, pull-down. (C) Atg37 is an integral membrane protein. PNS, postnuclear supernatant; 27S, 27,000 g supernatant; 27P, 27,000 g pellet; S, 200,000 g supernatant; P, 200,000 g pellet. (D) Atg37 localizes to the peroxisomal membranes (in WT) or ER-associated peroxisome remnants labeled with Pex3-mRFP (in Δpex19). DIC, differential interference contrast. Bar, 5 µm. (E) The TMD is essential for peroxisomal localization of Atg37. SKL, Ser-Lys-Leu, a peroxisomal targeting signal. Bar, 5 µm.

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