Figure 5. Accumulation and depletion of flagellar proteins in wild-type and dhc1b-3 cells at 21 and 34°C. (A) Immunofluorescence images of wild-type cells at 21°C (left column), dhc1b-3 cells at 21°C (middle column), and dhc1b-3 cells after 18 h at 34°C (right column), showing the relationship between dynein depletion and IFT protein accumulation. The top merged images are DHC1b (green) and IFT81 (red). The bottom merged images are D1bLIC (green) and IFT139 (red). Bar, 5 µm. (B) Western blots of flagella isolated from wild-type and dhc1b-3 cells at 21°C and increasing time at 34°C, as indicated. Dynein depletion correlated with the accumulation of IFT-B and IFT-A proteins, while the levels of kinesin, BBSome, and many axonemal proteins are unaffected (quantified in Table S2).
Figure 5.

Accumulation and depletion of flagellar proteins in wild-type and dhc1b-3 cells at 21 and 34°C. (A) Immunofluorescence images of wild-type cells at 21°C (left column), dhc1b-3 cells at 21°C (middle column), and dhc1b-3 cells after 18 h at 34°C (right column), showing the relationship between dynein depletion and IFT protein accumulation. The top merged images are DHC1b (green) and IFT81 (red). The bottom merged images are D1bLIC (green) and IFT139 (red). Bar, 5 µm. (B) Western blots of flagella isolated from wild-type and dhc1b-3 cells at 21°C and increasing time at 34°C, as indicated. Dynein depletion correlated with the accumulation of IFT-B and IFT-A proteins, while the levels of kinesin, BBSome, and many axonemal proteins are unaffected (quantified in Table S2).

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