Figure 5. GTPase-inactive short OPA1 forms colocalize with sites of mitochondrial division. (A) S-OPA1K301A-Flag expressed in Yme1l−/−Oma1−/− cells assembles into punctae partially colocalizing with ER–mitochondria contact sites (i; a total of 90 spots in five different cells), endogenous DRP1 (ii; a total of 230 spots in five different cells), and endogenous MID49 (iii; a total of 186 spots in six different cells). Bars, 2 µm. (B) Line scan of ii and iii along the lines indicated in A. (C) S-OPA1K301A-Flag punctae coalesce at sites of mitochondria constriction. Mitochondria are visualized using mito-mCherry, the ER using GRP78-specific antibodies. Bar, 2 µm.
Figure 5.

GTPase-inactive short OPA1 forms colocalize with sites of mitochondrial division. (A) S-OPA1K301A-Flag expressed in Yme1l−/−Oma1−/− cells assembles into punctae partially colocalizing with ER–mitochondria contact sites (i; a total of 90 spots in five different cells), endogenous DRP1 (ii; a total of 230 spots in five different cells), and endogenous MID49 (iii; a total of 186 spots in six different cells). Bars, 2 µm. (B) Line scan of ii and iii along the lines indicated in A. (C) S-OPA1K301A-Flag punctae coalesce at sites of mitochondria constriction. Mitochondria are visualized using mito-mCherry, the ER using GRP78-specific antibodies. Bar, 2 µm.

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