Figure 2.

Plakoglobin is present in skeletal muscle. (A) Paraffin-embedded longitudinal and cross sections of TA muscles from fed mice (top; bar, 20 µm), and C2C12 myotubes (bottom; bar, 15 µm) were stained with anti-plakoglobin. (B) Plakoglobin is present in the cytosolic and membrane fractions of muscle. 0.25% of membrane fraction and 0.01% of cytosolic fraction were analyzed by immunoblotting. GAPDH serves as a cytosolic marker. (C) Plakoglobin is present on the muscle membrane. Paraffin-embedded cross sections of normal TA muscles were stained with anti-plakoglobin and anti-dystrophin. Bar, 20 µm. The box on the right indicates an area of magnification (bar, 4.3 µm). (D) Plakoglobin is present in satellite cells. Paraffin–embedded cross sections of normal TA muscles were stained with anti-plakoglobin and anti-pax7 antibodies. Bar, 12 µm.

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