Microinjection of mitotic cells with anti-H3T3ph compromises the spindle checkpoint response in combination with Aurora B inhibition. (A) LLC-PK expressing EGFP-topoisomerase IIα arrested in mitosis with 0.17 µM (left) or 3.3 µM nocodazole (right) were injected with anti-H3T3ph solution containing Dextran Texas red, and time-lapse phase contrast and fluorescence images were collected every 15 min. Note the nuclear reformation at the last time point in each case. Times are in hours:minutes. Bar, 10 µm. (B–E) After treatment with the nocodazole and ZM447439 combinations indicated, exit from mitosis or death in mitosis was enumerated from live imaging series collected over 15 h as in A.