Haspin inhibitors delay BubR1 recruitment to kinetochores. (A) Immunofluorescence microscopy of BubR1 during Aurora B reactivation in the presence or absence of Haspin inhibitors in HeLa cells treated as in Fig. 4 A, but using 5 µM nocodazole throughout. Bar, 5 µm. (B) Approximately 100 mitotic cells in each condition from A were classified according to BubR1 intensity at kinetochores. Means ± SD are shown (error bars), n = 3. (C) HeLa cells were transfected with plasmids encoding EGFP–CENP-B or CENP-B–INCENP–EGFP between and after double thymidine treatments, and then treated essentially as in A. Bars, 5 µm. (D) For cells in C, the intensity of kinetochore BubR1 was classified in at least 100 cells per condition in one experiment. Similar results were obtained in a duplicate experiment.