Visualization of adhesion complexes using vinculin staining in CYK4 GAP-defective cells in anaphase. HeLa cells expressing inducible EGFP-CYK4 or GAP activity–defective CYK4^R385A were transfected with control or CYK4 3′-UTR siRNA duplexes in combination with siRNA duplexes targeting Rac1-specific effectors. CYK4 expression was induced for 60 h, and the cells were fixed and then stained with antibodies to GFP to detect CYK4 and vinculin and DAPI to detect DNA. Arrows indicate especially bright vinculin spots. The vinculin staining intensity was measured using ImageJ and is plotted in the bar graph. Error bars indicate the standard deviation from the means (n = 7). siControl, nonsilencing control; WT, wild type. Bars, 10 µm.