Figure 1. Stoichiometries and molecular... | Rockefeller University Press

$Figure 1. Stoichiometries and molecular masses of SHE subcomplexes. (A) Isothermal titration calorimetry with She3p titrated into Myo4p revealed a stoichiometry of 1.93 ± 0.20 (mean and standard deviation of two experiments) for the She3p:Myo4p complex. (B) Quantitative mass spectrometry with heavy isotope-labeled peptides showed a stoichiometry of 2.56 ± 0.11 (mean and standard deviation of two independent experiments) for the She3p:Myo4p complex. The amount of She3p was normalized with respect to Myo4p. (C) SLS profile of Myo4p shows that this protein is monomeric. RI, refractive index; RALS, right angle light scattering; MM, fitted molecular mass. (D) SLS profile of the She3p:Myo4p complex. Note that a slight excess of Myo4p was used to ensure saturation of the complex. (E) SLS profile of the ASH1-E3-77-tRNA:She2p complex.$

Figure 1.

Stoichiometries and molecular masses of SHE subcomplexes. (A) Isothermal titration calorimetry with She3p titrated into Myo4p revealed a stoichiometry of 1.93 ± 0.20 (mean and standard deviation of two experiments) for the She3p:Myo4p complex. (B) Quantitative mass spectrometry with heavy isotope-labeled peptides showed a stoichiometry of 2.56 ± 0.11 (mean and standard deviation of two independent experiments) for the She3p:Myo4p complex. The amount of She3p was normalized with respect to Myo4p. (C) SLS profile of Myo4p shows that this protein is monomeric. RI, refractive index; RALS, right angle light scattering; MM, fitted molecular mass. (D) SLS profile of the She3p:Myo4p complex. Note that a slight excess of Myo4p was used to ensure saturation of the complex. (E) SLS profile of the ASH1-E3-77-tRNA:She2p complex.

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