Identification and characterization of proteins in various nuclear subcompartments. (A) Overall schematic flow of this protein localization screen. (B) Representative images of proteins that show colocalization with various nuclear body markers. Bar, 10 µm. (C) The pie chart shows the distribution of proteins in various nuclear subcompartments (148 nucleolar proteins were not included). A total of 325 proteins that formed nuclear foci were identified in this screen, including 148 in the nucleolus, 38 in PML bodies, 27 in nuclear speckles, 24 in paraspeckles, 17 in Cajal bodies, 5 in Sam68 nuclear bodies, 2 in Polycomb bodies, and 64 proteins in uncharacterized nuclear subcompartments (please also see Table S1).