Figure 7. Tracking of each individual TfR molecule on the plasma membrane of live NRK cells, using mSiNC-Tf. (A and B) Single-particle images of mSiNC-Tf (human Tf used throughout this study), showing the binding and nonbinding of mSiNC-Tf to the bottom surfaces of a single NRK cell (arrowheads; see Video 2; A) and a single CEF cell (B), respectively. Human Tf has been known to bind to rat TfR, but not chick TfR, and thus, B serves as a negative control for A. (C) A typical trajectory (∼122 s and 3,651 frames) of a TfR molecule tagged by an mSiNC-Tf, observed in the bottom plasma membrane of an NRK cell at video rate (33 ms/frame). (D) A typical trajectory (5 s and 150 frames) of a TfR molecule conjugated with Cy3-Tf, obtained as in D. (E) The distribution of the diffusion coefficient of TfR in the time scale of 100 ms, determined with mSiNC-Tf, is similar to that determined with Cy3-Tf. The numbers in the box indicate median values (see arrowheads).
Figure 7.

Tracking of each individual TfR molecule on the plasma membrane of live NRK cells, using mSiNC-Tf. (A and B) Single-particle images of mSiNC-Tf (human Tf used throughout this study), showing the binding and nonbinding of mSiNC-Tf to the bottom surfaces of a single NRK cell (arrowheads; see Video 2; A) and a single CEF cell (B), respectively. Human Tf has been known to bind to rat TfR, but not chick TfR, and thus, B serves as a negative control for A. (C) A typical trajectory (∼122 s and 3,651 frames) of a TfR molecule tagged by an mSiNC-Tf, observed in the bottom plasma membrane of an NRK cell at video rate (33 ms/frame). (D) A typical trajectory (5 s and 150 frames) of a TfR molecule conjugated with Cy3-Tf, obtained as in D. (E) The distribution of the diffusion coefficient of TfR in the time scale of 100 ms, determined with mSiNC-Tf, is similar to that determined with Cy3-Tf. The numbers in the box indicate median values (see arrowheads).

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