Figure 5. The nonphosphorylated form of BubR1 reduces Aurora B–mediated Ndc80 phosphorylation at kinetochores. (A) Immunolocalization of mCherry-BubR1, ACA, and pS55-Hec1 in cells expressing wild-type (WT) BubR1 or BubR1 mutants as indicated. Bar, 10 µm. (B) Quantification of the normalized integrated intensities of the kinetochore signals of pS55-Hec1/ACA (±SD; the number of kinetochores was analyzed from ≥10 different cells as shown with three repeated experiments; *, P < 0.05). (C) Hec1 kinetochore localization is not affected in cells expressing BubR1 phosphorylation mutants. Immunolocalization of mCherry-BubR1 and Hec1 as indicated.
Figure 5.

The nonphosphorylated form of BubR1 reduces Aurora B–mediated Ndc80 phosphorylation at kinetochores. (A) Immunolocalization of mCherry-BubR1, ACA, and pS55-Hec1 in cells expressing wild-type (WT) BubR1 or BubR1 mutants as indicated. Bar, 10 µm. (B) Quantification of the normalized integrated intensities of the kinetochore signals of pS55-Hec1/ACA (±SD; the number of kinetochores was analyzed from ≥10 different cells as shown with three repeated experiments; *, P < 0.05). (C) Hec1 kinetochore localization is not affected in cells expressing BubR1 phosphorylation mutants. Immunolocalization of mCherry-BubR1 and Hec1 as indicated.

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