Density maps of pericentric LacO show differences in the position probability. (A) The mean position of WT pericentric LacO 1.7 kb from CEN 3, LacO 6.8 kb from CEN 15, and 8.8 kb from CEN 3 was determined in metaphase spindles by mapping the peak intensity of the LacO relative to the spindle pole body (red circles). The number and position of peak LacO intensity were used to generate a color-coded heat map of pericentric chromatin position in the spindle. (B) Position probability of WT pericentric LacO 6.8 kb displaying a stretched line signal. An overlay of WT 6.8 kb foci (green) and WT 6.8 kb stretching (red) heat maps allows for comparison. (C) Position probability of pericentric LacO 6.8 kb is altered distinctly in Gal-H3, mcm21Δ, and brn1-9. An overlay of mcm21Δ (green) and brn1-9 (red) heat maps allows for comparison. (A–C) Vertical white brackets represent mean diameter, and horizontal white brackets represent the mean distance from the spindle pole (values in Table I). (D) Graph of LacO distance from centromere in base pairs versus the distance from the pole in nanometers (WT LacO, 1.7, 6.8, and 8.8 kb [◆, black line]; and WT stretched, 6.8 kb [◇, gray line]). (E) A bar graph of mean diameter displacement perpendicular to the spindle axis for the LacO array 6.8 kb from the centromere in stretched, WT, Gal-H3, brn1-9, and mcm21Δ. The error bars represent SEM. The data and number of experiments for D and E are summarized in Table I.