Increased stretching of pericentric chromatin LacO spots in pericentric cohesin and condensin mutants. (A) Pericentric LacO 6.8 kb from CEN 15 images were classified as one focus, two foci, one focus and one stretched, two stretched, and two foci at one pole. The percentages of cells displaying each class of LacO fluorescence for each strain background are graphed with representative images of each class to the right (WT: 24 ± 4% single focus, 65 ± 0% two foci, 11 ± 4% one stretched, three experiments, n = 167; Gal-H3: 13 ± 3% single focus, 68 ± 1% two foci, 19 ± 2% one stretched, two experiments, n = 89; mcm21Δ: 11 ± 1% single focus, 36 ± 3% two foci, 47 ± 5% one stretched, 5 ± 0% two stretched, two experiments, n = 106; brn1-9: 6 ± 5% single focus, 38 ± 2% two foci, 52 ± 2% one stretched, 3 ± 1% two stretched, two experiments, n = 83; mcd1-1: 82% two foci, 18% two foci at one pole, one experiment, n = 17). The error bars represent SD. Bar, 2 µm. (B) The percentage of total cells compiled from all experiments displaying each class of LacO fluorescence is binned by spindle length into bins of 0.5 µm and graphed for WT, Gal-H3, mcm21Δ, and brn1-9. The data are the same for those described in A. Means and SEM values are listed in Table S1.