Summary of protein label position along the kMT axis for 26 epitopes in 23 kinetochore proteins in control and Spindly motif mutant (SpindlyF258A)–expressing cells. (A) The positions of the newly measured proteins/epitopes obtained from mean values of Delta measurements in Table 1, whereas the previously published measurements are from Wan et al. (2009). Scale on the left side is set to 0 at the position of CENP-I; positive values are outward (toward the spindle microtubules), whereas negative values are inward (toward the centromeric chromatin). Color-coded boxes indicate complexes and large proteins. Black dots indicate the average Delta values that have been corrected for tilt. The vertical lines indicate the minimum and maximum Delta values measured across the variation in centromere stretch. (B) A schematic view of kinetochore protein architecture for the RZZ and Mad1–Mad2 checkpoint protein module proteins relative to the KMN network for metaphase spindly motif mutant cells based on measurements in A. K-K, interkinetochore; N, N terminus; C, C terminus.
