Diaphanous and ROCK regulate the actomyosin flow. (Ai) Movie stills of a pupal notum expressing UAS-mCherry-Moesin driven by pnr-GAL4 show the flow of increasing actin concentration, which is visible in the early minutes of wound healing. Both Dia and ROCK RNAi impair the actin flow, leading to a weaker cable. (Aii) Graphic representation of the actin flow, color coded as Fig. 2 Aii. An impairment of the actin flow can be seen in the cases of Dia and ROCK RNAi. Bar, 10 µm. (Bi) Graph representing the variation of actin flow intensity in WT, Dia RNAi, and ROCK RNAi. Actin flow intensity decreases when expression of either gene is reduced. (Bii) Graph representing the variation of myosin flow intensity in WT, Dia RNAi, and ROCK RNAi. Myosin flow intensity decreases when expression of either gene is down-regulated. ROCK knockdown is particularly effective in reducing myosin concentration. Shadows represent the SEM for each curve. (Biii) Quantification of myosin cable intensity after wounding in WT, Dia RNAi–, and ROCK RNAi–expressing tissues reveals that the cable is weaker when Dia or ROCK expression is reduced (P = 0.008 and P = 0.004, respectively; Mann-Whitney test).