Figure 7.
Figure 7. Coordination of NTD and TACC domains in tandem allows binding to MTs. (A) Schematic diagram of clACC to show tandem NTD-TACC domains. (B) Representative confocal micrographs of GFP-clACC, GFP-CHC, or GFP-TACC3 expressed in HeLa cells. Cells were fixed and stained for pericentrin (red), tubulin, and DNA (blue). (C) Bar graph of spindle recruitment for each construct. Values are mean ± SEM calculated from Ncell = 52–81 over three experiments. ***, P < 0.001 from a Kruskal-Wallis test with Dunn’s multiple comparisons test. (D) Representative confocal micrographs of GFP-clACC in interphase HeLa cells stained for tubulin. Bars, 10 µm.

Coordination of NTD and TACC domains in tandem allows binding to MTs. (A) Schematic diagram of clACC to show tandem NTD-TACC domains. (B) Representative confocal micrographs of GFP-clACC, GFP-CHC, or GFP-TACC3 expressed in HeLa cells. Cells were fixed and stained for pericentrin (red), tubulin, and DNA (blue). (C) Bar graph of spindle recruitment for each construct. Values are mean ± SEM calculated from Ncell = 52–81 over three experiments. ***, P < 0.001 from a Kruskal-Wallis test with Dunn’s multiple comparisons test. (D) Representative confocal micrographs of GFP-clACC in interphase HeLa cells stained for tubulin. Bars, 10 µm.

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