Figure 1.
Figure 1. Two regions in TACC3 are needed for spindle recruitment: the TACC domain and the CID comprising a dileucine motif and pS558. (A) Representative confocal micrographs of GFP-TACC3 constructs (green) coexpressed in TACC3-depleted HeLa cells with mCherry-LCa (red) and DNA (blue). (bottom) Schematic of truncation constructs. (B) Alignment of the CID from TACC3 homologues to show sequence conservation. (C) Representative confocal micrographs as in A, for TACC3 point mutants. Bars, 10 µm.

Two regions in TACC3 are needed for spindle recruitment: the TACC domain and the CID comprising a dileucine motif and pS558. (A) Representative confocal micrographs of GFP-TACC3 constructs (green) coexpressed in TACC3-depleted HeLa cells with mCherry-LCa (red) and DNA (blue). (bottom) Schematic of truncation constructs. (B) Alignment of the CID from TACC3 homologues to show sequence conservation. (C) Representative confocal micrographs as in A, for TACC3 point mutants. Bars, 10 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal