Figure 8.
Figure 8. Exo84 phosphorylation inhibits daughter cell surface expansion. (A) The growth of the wild-type and exo84 phosphorylation mutants coexpressing Sec5-3×GFP and Nup57-MARS (a nuclear envelope marker) was monitored by time-lapse microscopy (see Fig. S4 for the time-lapse images). Representative images of cells at the end of mitosis as indicated by relocalization of Sec5-3×GFP to the bud neck are shown. Anaphase and metaphase phase were determined by nuclear morphology using Nup57-MARS as a marker. DIC, differential interference contrast. Bars, 2.5 µm. (B) The ratios of daughter cell (“bud”) to mother cell surface areas at late mitotic phase were plotted for each group. Error bars represent standard error of the mean. *, P < 0.01; n > 100.

Exo84 phosphorylation inhibits daughter cell surface expansion. (A) The growth of the wild-type and exo84 phosphorylation mutants coexpressing Sec5-3×GFP and Nup57-MARS (a nuclear envelope marker) was monitored by time-lapse microscopy (see Fig. S4 for the time-lapse images). Representative images of cells at the end of mitosis as indicated by relocalization of Sec5-3×GFP to the bud neck are shown. Anaphase and metaphase phase were determined by nuclear morphology using Nup57-MARS as a marker. DIC, differential interference contrast. Bars, 2.5 µm. (B) The ratios of daughter cell (“bud”) to mother cell surface areas at late mitotic phase were plotted for each group. Error bars represent standard error of the mean. *, P < 0.01; n > 100.

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