Figure 7. RhoGEF2 negatively regulates mobile E-cad–Baz. (A–D) Endogenous E-cad (A; black/green, anti–E-cad) and Baz (C; black/green, anti-Baz) localization in wild type and upon RhoGEF2 overexpression or down-regulation with RhoGEF2-RNAi (A and C; magenta), and quantitation of E-cad (B) and Baz (D) junctional levels. Bars, 5 µm. (E–H) E-cad–GFP FRAP upon RhoGEF2 overexpression or down-regulation with RhoGEF2-RNAi at DV (E and F) and AP borders (G and H). Examples of recovery are shown in E and G, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) in F and H. (I) Combining this data provides an estimate of mobile and immobile E-cad pools (error bars indicate mean ± SEM) at DV and AP borders. *, P < 0.01; **, P < 0.001; ***, P < 0.0001.
Figure 7.

RhoGEF2 negatively regulates mobile E-cad–Baz. (A–D) Endogenous E-cad (A; black/green, anti–E-cad) and Baz (C; black/green, anti-Baz) localization in wild type and upon RhoGEF2 overexpression or down-regulation with RhoGEF2-RNAi (A and C; magenta), and quantitation of E-cad (B) and Baz (D) junctional levels. Bars, 5 µm. (E–H) E-cad–GFP FRAP upon RhoGEF2 overexpression or down-regulation with RhoGEF2-RNAi at DV (E and F) and AP borders (G and H). Examples of recovery are shown in E and G, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) in F and H. (I) Combining this data provides an estimate of mobile and immobile E-cad pools (error bars indicate mean ± SEM) at DV and AP borders. *, P < 0.01; **, P < 0.001; ***, P < 0.0001.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal