EB1-DN and Spas reduce mobile E-cad–Baz and its asymmetry. (A–D) Endogenous E-cad (A) and Baz (C) localization in cells expressing EB1-DN or Spas (anti–E-cad, black/green in A; anti-Baz, black/green in C; Cherry-tagged products in experimental cells, magenta), and quantitation of E-cad (B) and Baz (D) levels at the junctions. Bars, 5 µm. (E–H) Recovery of E-cad–GFP in cells that express CD8, EB1-DN, or Spas at DV borders (E and F) and AP borders (G and H). Examples of recovery are shown in E and G, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) in F and H. wt, wild-type cells adjacent to experimental. (G) Combining this data provides an estimate of mobile and immobile E-cad pools (mean ± SEM); inhibition of MTs especially reduced the mobile E-cad at DV borders. Fig. S2 shows the same defect in Eb1 mutant embryos. **, P < 0.001; ***, P < 0.0001.