Mechanisms of Baz recovery. (A–D) Baz-GFP FRAP at DV borders. Baz-GFP recovery is via diffusion and trafficking, similar to E-cad–GFP: comparing 1-µm and 2-µm bleach spots with the inset in B showing a closer view of the initial recovery to highlight the difference (A and B), and in cells expressing ShiDN (C and D). Examples of recovery are shown in A and C, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) with best fit curves shown as solid lines in B and D. (E) E-cad and Baz-GFP colocalized in tubular structures (arrows) caused by expression of Shi-DN. Colocalization is shown in white in right image. Bar, 5 µm.