Figure 2. Mechanisms of E-cad recovery. (A and B) Localization of Echinoid-YFP (A; Ed, black/green in epidermal cells) and quantification (B) of Ed junctional levels in cells expressing EB1-DN (magenta in A) and adjacent wild-type cells. Bar, 5 µm. (C–H) E-cad–GFP FRAP recovery at DV borders: (C–D) comparing 1-µm and 2-µm bleach spots with the inset in D showing a closer view of the initial recovery to highlight the difference; (E and F) with ShiDN versus wild type; and (G and H) comparing the first and second bleach of the same spot. Examples of recovery are shown in C, E, and G, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) with best fit curves shown as solid lines in D, F, and H. The first frame after the first bleach event (B) and the prebleach frame before the second bleach event (S) are shown in G.
Figure 2.

Mechanisms of E-cad recovery. (A and B) Localization of Echinoid-YFP (A; Ed, black/green in epidermal cells) and quantification (B) of Ed junctional levels in cells expressing EB1-DN (magenta in A) and adjacent wild-type cells. Bar, 5 µm. (C–H) E-cad–GFP FRAP recovery at DV borders: (C–D) comparing 1-µm and 2-µm bleach spots with the inset in D showing a closer view of the initial recovery to highlight the difference; (E and F) with ShiDN versus wild type; and (G and H) comparing the first and second bleach of the same spot. Examples of recovery are shown in C, E, and G, with red circles on the prebleached frame (P) showing the bleach spots, and averaged recovery curves (error bars indicate mean ± SEM) with best fit curves shown as solid lines in D, F, and H. The first frame after the first bleach event (B) and the prebleach frame before the second bleach event (S) are shown in G.

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