RNF111 promotes NER by regulating XPC recruitment to UV-damaged DNA. (A) UDS of the indicated MEF cell lines, determined by EdU incorporation for 3 h after exposure to 16 J/m2 UV-C. Error bars indicate SDs of three independent experiments. (B) Cells stably expressing XPC-GFP were transfected with indicated siRNAs and locally exposed to laser-induced UV-C damage. XPC-GFP fluorescence intensity at the damaged area relative to predamage intensity was recorded in time using live-cell confocal imaging (mean of three independent experiments, n = 8 cells per experiment, ±SD). (C) As in B, except that cells were transfected with control (CTRL) or DDB2 siRNA. Results of a representative experiment (n = 8 cells per sample, ±SEM) are shown.
