Figure 5.

Mutating the miR-129 seed match sequence in EGFP-Kv1.1 RNA increases protein levels without changing RNA levels. Representative images of dendritic localization of EGFP-Kv1.1 RNA (red) and protein (green) with the intact (left, FL) or mutated (right, ΔmiR-129) miR-129 binding site revealed by in situ hybridization (ISH) using a digoxigenin-labeled antisense oligo against EGFP. Bar, 20 µM. n = 12 and 13 neurons for FL and ΔmiR-129, 40 dendrites each. Error bars show SEMs.

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